Communications: Comparison of Traditional and Molecular Detection Methods forBaculovirus penaeiInfections in LarvalPenaeus vannamei

1994 ◽  
Vol 6 (4) ◽  
pp. 355-359 ◽  
Author(s):  
Linda D. Bruce ◽  
Donald V. Lightner ◽  
Rita M. Redman ◽  
Kenneth C. Stuck
Keyword(s):  
Molecular Detection ◽  
Detection Methods

scholarly journals Ebolavirus diagnosis made simple, comparable and faster than molecular detection methods: preparing for the future

2018 ◽  
Vol 15 (1) ◽  
Author(s):  
Ameh S. James ◽  
Shawn Todd ◽  
Nina M. Pollak ◽  
Glenn A. Marsh ◽  
Joanne Macdonald
Keyword(s):  
Molecular Detection ◽  
Detection Methods ◽  
The Future

scholarly journals Molecular Detection Methods for Vibrio parahaemolyticus in Seafood

2015 ◽  
Vol 89 (4) ◽  
pp. 445-451 ◽  
Author(s):  
Tomohiro NISHIO ◽  
Kayoko OHTSUKA ◽  
Midori ODA ◽  
Kanji SUGIYAMA ◽  
Yukiko HARA-KUDO
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Molecular Detection ◽  
Detection Methods

Molecular detection methods of human papillomavirus (HPV)

2009 ◽  
Vol 24 (4) ◽  
pp. 215-222 ◽  
Author(s):  
Apostolos Zaravinos ◽  
Ioannis N. Mammas ◽  
George Sourvinos ◽  
Demetrios A. Spandidos
Keyword(s):  
Human Papillomavirus ◽  
Molecular Detection ◽  
Detection Methods

scholarly journals Non-cultural detection and molecular genotyping of Neisseria gonorrhoeae from a piece of clothing

2007 ◽  
Vol 56 (4) ◽  
pp. 487-490 ◽  
Author(s):  
Iona M. C. Martin ◽  
Ellie Foreman ◽  
Vicky Hall ◽  
Anne Nesbitt ◽  
Greta Forster ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Molecular Detection ◽  
Definitive Diagnosis ◽  
Sequence Type ◽  
Detection Methods ◽  
Legal Cases ◽  
Immunological Tests ◽  
The Uk ◽  
Relationship Of ◽  
The Relationship

Isolation of Neisseria gonorrhoeae is currently the gold standard for the definitive diagnosis of gonorrhoea and for use in medico-legal cases in the UK. Molecular detection methods are used increasingly but are untested as evidence of infection in a court of law. An isolate of N. gonorrhoeae was obtained from a child and an article of clothing from an adult male who was suspected of sexual abuse of the child. Biochemical and immunological tests were used to confirm the isolate as N. gonorrhoeae. Amplification by PCR using two targets, cppB and ompIII, was used both as further confirmation of the isolate and to detect the presence of gonococcal-specific DNA from the clothing. The relationship of the gonococcal DNA from the child and the adult was investigated using genotyping (N. gonorrhoeae multi-antigen sequence typing; NG-MAST), including a nested PCR for the por gene. Both samples were indistinguishable by NG-MAST and shared the same sequence type, 403. This is the first report of molecular detection and genotyping of N. gonorrhoeae on an article of clothing, which resulted in conviction of the man for sexual assault.

Comparison of three molecular detection methods for detection of Trichinella in infected pigs

2013 ◽  
Vol 112 (5) ◽  
pp. 2087-2093 ◽  
Author(s):  
Zhibing Lin ◽  
Jie Cao ◽  
Houshuang Zhang ◽  
Yongzhi Zhou ◽  
Mingjun Deng ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Detection Methods

scholarly journals Epidemiology of clinically relevant Entamoeba spp. (E. histolytica/dispar/moshkovskii/bangladeshi): A cross sectional study from North India

2021 ◽  
Vol 15 (9) ◽  
pp. e0009762
Author(s):  
Aradhana Singh ◽  
Tuhina Banerjee ◽  
Uzma Khan ◽  
Sunit Kumar Shukla
Keyword(s):  
Molecular Detection ◽  
Cross Sectional Study ◽  
North India ◽  
Detection Methods ◽  
Cross Sectional ◽  
The People ◽  
Intestinal Infections ◽  
Routine Identification ◽  
Stool Samples ◽  
The City

Background Entamoeba infections have major impact on millions of the people worldwide. Entamoeba histolytica has long been accepted as the only pathogenic species. However, recent reports of other Entamoeba spp. in symptomatic cases have raised questions on their pathogenicity. Methodology/Principal findings Total 474 stool samples and 125 liver aspirates from patients with intestinal and extra intestinal manifestations and from community were included. Sewage samples from the hospital and the city were also included. Microscopic examination and molecular detection were performed to detect presence of E. histolytica/ dispar/ moshkovskii/ bangladeshi. The associated demographic and socioeconomic factors were statistically analyzed with the presence of Entamoeba. Microscopy detected Entamoeba spp. in 5.4% stool and 6.4% liver aspirate samples. Through nested multiplex PCR, prevalence of Entamoeba spp. in intestinal and extra-intestinal cases was 6.6% (20/301) and 86.4% (108/125) respectively and in asymptomatic population was 10.5% (13/123). Sewage samples did not show presence of any Entamoeba spp. Uneducated subjects, low economic conditions, untreated drinking water, consumption of raw vegetables and habit of not washing hands before meals were significantly associated with presence of Entamoeba spp. Conclusions E. histolytica still remains the only Entamoeba spp. in invasive extra intestinal infections. E. dispar was detected in both asymptomatic and symptomatic intestinal infections. Routine identification of Entamoeba spp. should incorporate PCR based detection methods.

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